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dc.contributor.authorCevik, Mehtap
dc.contributor.authorGunduz, Meliha Koldemir
dc.contributor.authorDeliorman, Gokce
dc.contributor.authorSusleyici, Belgin
dc.date.accessioned2021-03-15T13:06:10Z
dc.date.available2021-03-15T13:06:10Z
dc.date.issued2020
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.urihttps://doi.org/10.1007/s11033-020-05992-5
dc.identifier.urihttps://hdl.handle.net/20.500.12879/107
dc.descriptionWOS:000589439000001en_US
dc.descriptionPubMed: 33185830en_US
dc.description.abstractAdipocyte death is important in obesity development. Understanding and prevention of adipocyte deaths may be a molecular approach in the treatment. In the study, we aimed to understand role of Niban gene, which acts as an anti-apoptotic molecule as a response to stress conditions, in adipocytes. 3T3-L1 adipocytes were treated with different doses of linoleic acid, hydrogen peroxide and ethanol; and proliferation of the cells examined with real time monitoring iCELLingence system. Gene expression levels were measured by q-PCR. As a response to 24h 480 mu M linoleic acid treatment, Niban gene expression was found to be higher than control group (p = 0.008), whereas 24 h 90 mM ethanol treatment was determined to be lower than control group (p = 0.008). The highest value of Niban gene expression among H2O2 treatment groups was detected in 4h 600 mu M H2O2 in comparison to control group (p = 0.008). To understand role of Niban in adipogenesis, Niban gene expressions were compared between pre-adipocytes and advanced fat accumulated adipocytes and determined to be significantly different (p = 0.042). Our results suggest that Niban might be involved in stress response process in adipocytes. However, the exact molecular role of Niban needs to be investigated in further studies.en_US
dc.description.sponsorshipMarmara University Scientific Research Projects Coordination UnitMarmara University [FEN-C-YLP-080415-0125]; Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)en_US
dc.description.sponsorshipThis study was supported by Marmara University Scientific Research Projects Coordination Unit. Project No: FEN-C-YLP-080415-0125. Additionally, we would like to thank the Scientific and Technological Research Council of Turkey (TUBITAK) for supporting education of young scientists (M. Cevik) with 2210-C master program.en_US
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectNibanen_US
dc.subjectLinoleic aciden_US
dc.subjectCell stressen_US
dc.subjectObesityen_US
dc.subjectAdipogenesisen_US
dc.titleAlterations in niban gene expression as a response to stress conditions in 3T3-L1 adipocytesen_US
dc.typearticleen_US
dc.contributor.departmentBeykoz Üniversitesi Mühendislik ve Mimarlık Fakültesien_US
dc.contributor.institutionauthorDeliorman, Gokce
dc.identifier.doi10.1007/s11033-020-05992-5
dc.identifier.volume47en_US
dc.identifier.issue12en_US
dc.identifier.startpage9399en_US
dc.identifier.endpage9408en_US
dc.relation.journalMolecular Biology Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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