dc.contributor.author | Cevik, Mehtap | |
dc.contributor.author | Gunduz, Meliha Koldemir | |
dc.contributor.author | Deliorman, Gokce | |
dc.contributor.author | Susleyici, Belgin | |
dc.date.accessioned | 2021-03-15T13:06:10Z | |
dc.date.available | 2021-03-15T13:06:10Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 0301-4851 | |
dc.identifier.issn | 1573-4978 | |
dc.identifier.uri | https://doi.org/10.1007/s11033-020-05992-5 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12879/107 | |
dc.description | WOS:000589439000001 | en_US |
dc.description | PubMed: 33185830 | en_US |
dc.description.abstract | Adipocyte death is important in obesity development. Understanding and prevention of adipocyte deaths may be a molecular approach in the treatment. In the study, we aimed to understand role of Niban gene, which acts as an anti-apoptotic molecule as a response to stress conditions, in adipocytes. 3T3-L1 adipocytes were treated with different doses of linoleic acid, hydrogen peroxide and ethanol; and proliferation of the cells examined with real time monitoring iCELLingence system. Gene expression levels were measured by q-PCR. As a response to 24h 480 mu M linoleic acid treatment, Niban gene expression was found to be higher than control group (p = 0.008), whereas 24 h 90 mM ethanol treatment was determined to be lower than control group (p = 0.008). The highest value of Niban gene expression among H2O2 treatment groups was detected in 4h 600 mu M H2O2 in comparison to control group (p = 0.008). To understand role of Niban in adipogenesis, Niban gene expressions were compared between pre-adipocytes and advanced fat accumulated adipocytes and determined to be significantly different (p = 0.042). Our results suggest that Niban might be involved in stress response process in adipocytes. However, the exact molecular role of Niban needs to be investigated in further studies. | en_US |
dc.description.sponsorship | Marmara University Scientific Research Projects Coordination UnitMarmara University [FEN-C-YLP-080415-0125]; Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) | en_US |
dc.description.sponsorship | This study was supported by Marmara University Scientific Research Projects Coordination Unit. Project No: FEN-C-YLP-080415-0125. Additionally, we would like to thank the Scientific and Technological Research Council of Turkey (TUBITAK) for supporting education of young scientists (M. Cevik) with 2210-C master program. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Springer | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Niban | en_US |
dc.subject | Linoleic acid | en_US |
dc.subject | Cell stress | en_US |
dc.subject | Obesity | en_US |
dc.subject | Adipogenesis | en_US |
dc.title | Alterations in niban gene expression as a response to stress conditions in 3T3-L1 adipocytes | en_US |
dc.type | article | en_US |
dc.contributor.department | Beykoz Üniversitesi Mühendislik ve Mimarlık Fakültesi | en_US |
dc.contributor.institutionauthor | Deliorman, Gokce | |
dc.identifier.doi | 10.1007/s11033-020-05992-5 | |
dc.identifier.volume | 47 | en_US |
dc.identifier.issue | 12 | en_US |
dc.identifier.startpage | 9399 | en_US |
dc.identifier.endpage | 9408 | en_US |
dc.relation.journal | Molecular Biology Reports | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |