PubMed İndeksli Yayınlar Koleksiyonu

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Alterations in Niban Gene Expression as a Response to Stress Conditions in 3t3-L1 Adipocytes
(Springer, 2020) Cevik, Mehtap; Gunduz, Meliha Koldemir; Deliorman, Gokce; Susleyici, Belgin
Adipocyte death is important in obesity development. Understanding and prevention of adipocyte deaths may be a molecular approach in the treatment. In the study, we aimed to understand role of Niban gene, which acts as an anti-apoptotic molecule as a response to stress conditions, in adipocytes. 3T3-L1 adipocytes were treated with different doses of linoleic acid, hydrogen peroxide and ethanol; and proliferation of the cells examined with real time monitoring iCELLingence system. Gene expression levels were measured by q-PCR. As a response to 24h 480 mu M linoleic acid treatment, Niban gene expression was found to be higher than control group (p = 0.008), whereas 24 h 90 mM ethanol treatment was determined to be lower than control group (p = 0.008). The highest value of Niban gene expression among H2O2 treatment groups was detected in 4h 600 mu M H2O2 in comparison to control group (p = 0.008). To understand role of Niban in adipogenesis, Niban gene expressions were compared between pre-adipocytes and advanced fat accumulated adipocytes and determined to be significantly different (p = 0.042). Our results suggest that Niban might be involved in stress response process in adipocytes. However, the exact molecular role of Niban needs to be investigated in further studies.
Applications of Exosome Vesicles in Different Cancer Types as Biomarkers
(Bentham Science Publishing Ltd, 2024) Ihlamur, Murat; Kelleci, Kubra; Zengin, Yagmur; Allahverdiyev, M. Adil; Abamor, Emrah Sefik
One of the biggest challenges in the fight against cancer is early detection. Early diagnosis is vital, but there are some barriers such as economic, cultural, and personal factors. Considering the disadvantages of radiological imaging techniques or serological analysis methods used in cancer diagnosis, such as being expensive, requiring expertise, and being time-consuming, there is a need to develop faster, more reliable, and cost-effective diagnostic methods for use in cancer diagnosis.Exosomes, which are responsible for intercellular communication with sizes ranging from 30-120 nm, are naturally produced biological nanoparticles. Thanks to the cargo contents they carry, they are a potential biomarker to be used in the diagnosis of cancer. Exosomes, defined as extracellular vesicles of endosomal origin, are effective in cancer growth, progression, metastasis, and drug resistance, and changes in microenvironmental conditions during tumor development change exosome secretion. Due to their high cellular activity, tumor cells produce much higher exosomes than healthy cells. Therefore, it is known that the number of exosomes in body fluids is significantly rich compared to other cells and can act as a stand-alone diagnostic biomarker. Cancer-derived exosomes have received great attention in recent years for the early detection of cancer and the evaluation of therapeutic response.In this article, the content, properties, and differences of exosomes detected in common types of cancer (lung, liver, pancreas, ovaries, breast, colorectal), which are the leading causes of cancer-related deaths, are reviewed. We also discuss the potential utility of exosome contents as a biomarker for early detection, which is known to be important in targeted cancer therapy.
Design and Simulation of the Microcantilever Biosensor for MITF Antigen and D5 Monoclonal Antibody Interaction Finite Element Analysis, and Experimental
(Bentham Science Publishing Ltd, 2024) Akcali, Pelin; Kelleci, Kubra; Ozer, Sevil
Background Biosensors and MEMS have witnessed rapid development and enormous interest over the past decades. Constant advancement in diagnostic, medical, and chemical applications has been demonstrated in several platforms and tools. In this study, the analytical and FEA of the microcantilever used in biomolecular analyses were compared with the experimental analysis results.Methods In this study, MITF antigen, which is a melanoma biomarker, and anti-MITF antibody (D5) were selected as biomolecules. A MEMS-type microcantilever biosensor was designed by functionalizing the AFM cantilever by utilizing the specific interaction dynamics and intermolecular binding ability between both molecules. Surface functionalization of cantilever micro biosensors was performed by using FEA. The stress that will occur as a result of the interactions between the MITF-D5 has been determined from the deviation in the resonant frequency of the cantilever.Results It has been found that the simulation results are supported by analytical calculations and experimental results.Conclusion The fact that the results of the simulation study overlap with the experimental and mathematical results allows us to get much cheaper and faster answers compared to expensive and time-consuming experimental approaches.
The Effects of Glipizide on DNA Damage and Nuclear Transport in Differentiated 3T3-L1 Adipocytes
(Springer, 2022) Cevik, Mehtap; Caker, Selen; Deliorman, Gokce; Cagatay, Penbe; Gunduz, Meliha Koldemir; Susleyici, Belgin
Background Despite commonly use for treatment of type II diabetes, possible effects of glipizide on nuclear transport and DNA damage in cells are unknown. Since clinical response of glipizide may change with aging, the aim of the study was to investigate the effect of glipizide by comparing mature and senescent adipocytes. Methods and results The effects of glipizide were investigated in 3T3-L1 adipocytes. Effective and lethal doses were determined by real-time monitoring iCELLigence system. Comet assay was performed to determine DNA damage and quantitative PCR was conducted to detect gene expression levels. RAN expressions were found to be up regulated in mature 180 mu M glipizide treated adipocytes compared to control group (p < 0.05); whereas down regulated in senescent 180 mu M glipizide treated adipocytes compared to their control adipocytes (p < 0.05). Olive Tail Moment values were significantly higher in mature 180 mu M glipizide treated adipocytes (MTG) and senescent 180 mu M glipizide treated adipocytes (STG) comparing their untreated controls (p < 0.001 and p < 0.001 respectively). Also class 5 comets that shows severe DNA damage were found to be higher in both MTG and STG groups than their controls (p < 0.001 and p < 0.001, respectively). OTM values were higher in STG than MTG (p < 0.001). Conclusions This is the first study that reports glipizide caused DNA damage increasing with senescence in adipocytes. As a response to glipizide treatment Ran gene expression increased in mature; and decreased in senescent adipocytes. Further studies are needed to reveal the effect of glipizide on DNA and nuclear interactions in molecular level.
Efficacy of Cognitive-Behavioral Therapy in Reducing Muscle Dysmorphia Symptoms Among Turkish Gym Goers: A Pilot Study
(Elsevier, 2024) Cinaroglu, Metin; Yilmazer, Eda; Ulker, Selami Varol; Ahlatcioglu, Esra Noyan; Sayar, Gokben Hizli
Muscle dysmorphia (MD) is a disorder marked by an obsessive focus on muscularity, often leading to excessive exercise, rigid dieting, and use of performance-enhancing drugs (PEDs). While Cognitive-Behavioral Therapy (CBT) is known to be effective for various body image disorders, its specific effects on individuals with MD have been less studied. This qualitative case series involved seven male participants, aged 35-55, all diagnosed with MD and with a history of anabolic steroid use, undergoing a 12-week manualized CBT intervention. The study aimed to explore how CBT impacts their body image perceptions, exercise behaviors, steroid use, and emotional responses. Data were collected through participant feedback during therapy sessions, with thematic analysis revealing significant shifts in body image perception and reductions in compulsive behaviors and steroid use. Despite these improvements, participants experienced feelings of loss and anxiety as they modified their routines, and some reported relapses under stress, indicating the chronic nature of MD. The findings suggest that CBT effectively reshapes cognitive and behavioral patterns in MD sufferers but also highlight the need for ongoing support to manage the disorder's emotional complexities and prevent relapses. Future research should focus on long-term and individualized therapeutic approaches to better support those with muscle dysmorphia.
Factors Affecting the Presence of Renal Cortical Cysts in Kidney Donors
(Aves, 2024) Cicek, Sevim Nuran Kuslu; Huseynov, Amil
Objective: Simple renal cysts (SRCs) represent the most frequently occurring type of renal cysts, frequently observed in the elderly population. While generally considered benign, SRCs may sometimes be connected to comorbid conditions such as hypertension, aortic diseases, and renal dysfunction. This research aims to investigate the factors influencing the development of SRCs in kidney donors and the associated risks. Methods: This retrospective cohort study included 1012 living kidney donors, aged 18-87 years, who underwent renal transplant donor nephrectomy between 2008 and 2023. Data on demographic information, cyst characteristics, comorbidities, and associated risk factors were collected and analyzed using statistical methods, including Binary Logistic Regression Analysis. Renal cysts were identified and classified using computed tomography (CT) and magnetic resonance imaging (MRI) methods. Results: Renal cortical cysts were more frequently observed in males (52.76%) compared to females (47.24%), with a significant difference (P = .031). Donors with renal cortical cysts were significantly older (mean age 54.43 +/- 12.17 years) compared to those without cysts (46.26 +/- 12.35 years, P < .001). Substantial differences were likewise noted in fasting blood glucose, uric acid, creatinine, HbA1c, and glomerular filtration rate (GFR).The prevalence of aortic atherosclerosis was notably elevated in donors with cysts (47.74%) compared to those without (23.57%, P < .001). Binary logistic regression analysis indicated that older age and being male were significant factors influencing the presence of cortical cysts. Conclusion: The study confirms that SRCs are the most common renal cyst type and are more frequently observed in the elderly population. While generally benign, SRCs may be associated with increased uric acid levels and other comorbidities, suggesting potential impacts on kidney health. Additional studies are required to investigate these associations. The presence of SRCs in kidney donors is significantly associated with male gender, age, uric acid levels, and creatinine levels. These findings should be considered during the evaluation of potential kidney donors, particularly regarding the associated risks and management of SRCs.
In Vitro Determination of Antileshmanial Activities of Benzimidazolium Derivatives on L. Major Promastigotes and Amastigotes
(Springer Int Publ Ag, 2023) Kelleci, Kubra; Golebatmaz, Eda
Purpose Leishmaniasis is a serious public health problem infecting millions of people worldwide. An effective and reliable treatment method to be used in the treatment of the disease has not been developed yet. Methods In this article, the anti-leishmanial activities of two benzimidazolium derivatives (B.A and B.B) against Leishmania major promastigotes and amastigotes, which are known to cause cutaneous leishmaniasis, were investigated for the first time. The immunostimulatory activity of the developed formulations was determined using the J774 murine macrophage cell line. Results B.A and B.B compounds were found to have a much higher cytotoxic effect than Amphotericin B (IC50 value 0.75 mu M +/- 0.03), which is used as the reference drug. The IC50 value was determined as 2.02 mu M +/- 0.52 for B.A and 1.83 mu M +/- 0.71 for B.B in Leishmania promastigotes. In addition, IC50 values of B. A and B.B Leishmania amastigotes were found to be 1.01 mu M and 0.67 mu M, respectively. It was found that B.B was 81.12 times more selective than Amphotericin B and showed the highest selectivity against L. major promastigotes (359.09) and amastigotes (980.80). Considering the selectivity indices (SI) of B.A and B.B, both compounds tested are more promising than Amphotericin B. Conclusion The results showed that benzimidazolium derivatives have anti-leishmanial potential against L. major, which is the causative agent of cutaneous leishmaniasis. Thus, we can say that the obtained results will help the development of effective and safe antileishmanial drug formulations against cutaneous leishmaniasis.
Laparoscopic Hand-Assisted Donor Nephrectomy: A Decade of Single-Center Experience and Outcomes
(AVES, 2024) Cicek, Sevim Nuran Kuslu; Huseynov, Amil
Objective: Kidney transplantation is the most effective treatment for end-stage renal disease, but the shortage of cadaveric donors has increased reliance on living donors. Traditional open donor nephrectomy, while effective, is associated with significant morbidity. Hand-assisted laparoscopic donor nephrectomy (HALDN) combines the advantages of minimally invasive surgery with the tactile feedback of open surgery. This study presents the outcomes of HALDN procedures performed at the center. Methods: A total of 1221 living donor nephrectomies performed between September 2009 and August 2021 at Medicana & Idot;stanbul Hospital were analyzed. Donor characteristics, surgical details, and postoperative outcomes were recorded. Data analysis was conducted using SPSS version 22.0, with continuous variables assessed for normality and expressed accordingly. Results: Donor ages ranged from 19 to 87 years (mean 48.50 +/- 12.75 years), with 54.8% female donors. The average body mass index (BMI) was 27.99 +/- 3.7 kg/m2. Left-sided nephrectomies constituted 78.5% of cases, with HALDN performed in 94.7% of these surgeries. Warm ischemia time averaged 99.21 +/- 56.67 seconds. The average blood loss was 70 mL, and the mean hospital stay was 4.12 +/- 1.2 days. Complications included conversion to open surgery due to bleeding in 3.3% of cases, postoperative atelectasis (0.6%), incisional hernia (0.33%), wound infection (0.16%), and scrotal swelling (0.25%). The findings indicate that HALDN is a safe and effective method for donor nephrectomy, aligning with similar studies regarding operation and warm ischemia times. The minimally invasive nature of HALDN contributes to shorter hospital stays and quicker postoperative recovery. The rate of conversion to open surgery was within acceptable limits, and complications were manageable. Obesity (BMI > 30 kg/m2) was identified as a risk factor for incisional hernia, suggesting the need for careful surgical technique in this group. Conclusion: Hand-assisted laparoscopic donor nephrectomy offers a minimally invasive, safe, and effective alternative for living donor nephrectomy, enhancing donor recovery and potentially encouraging organ donation. Its adoption may play a significant role in reducing the number of patients awaiting organ transplants.
The mTOR Signaling Pathway and mTOR Inhibitors in Cancer: Next-Generation Inhibitors and Approaches
(Bentham Science Publishing Ltd, 2024) Ihlamur, Murat; Akgul, Busra; Zengin, Yagmur; Korkut, Senay Vural; Kelleci, Kubra; Abamor, Emrah Sefik
mTOR is a serine/threonine kinase that plays various roles in cell growth, proliferation, and metabolism. mTOR signaling in cancer becomes irregular. Therefore, drugs targeting mTOR have been developed. Although mTOR inhibitors rapamycin and rapamycin rapalogs (everolimus, rapamycin, temsirolimus, deforolimus, etc.) and new generation mTOR inhibitors (Rapalink, Dual PI3K/mTOR inhibitors, etc.) are used in cancer treatments, mTOR resistance mechanisms may inhibit the efficacy of these drugs. Therefore, new inhibition approaches are developed. Although these new inhibition approaches have not been widely investigated in cancer treatment, the use of nanoparticles has been evaluated as a new treatment option in a few types of cancer.This review outlines the functions of mTOR in the cancer process, its resistance mechanisms, and the efficiency of mTOR inhibitors in cancer treatment. Furthermore, it discusses the next-generation mTOR inhibitors and inhibition strategies created using nanoparticles.Since mTOR resistance mechanisms prevent the effects of mTOR inhibitors used in cancer treatments, new inhibition strategies should be developed. Inhibition approaches are created using nanoparticles, and one of them offers a promising treatment option with evidence supporting its effectiveness.
Multimodal Neurophysiological Responses to Body Image Stimuli in Men with Muscle Dysmorphia and Steroid Use
(Elsevier, 2025) Cinaroglu, Metin; Yilmazer, Eda; Ulker, Selami Varol; Sayar, Gokben Hizli
Muscle dysmorphia (MD), a subtype of body dysmorphic disorder (BDD), involves a pathological preoccupation with muscularity and is commonly linked to anabolic steroid and performance-enhancing drugs (PEDs) use. Despite its rising prevalence, the neurophysiological and affective mechanisms underlying MD, particularly in steroid users, remain underexplored. This study examined 71 male strength-training participants (35 with BDD/ steroid use; 36 controls) during a passive viewing task of muscular, average, and slender male physiques while recording electroencephalography (EEG), electrodermal activity (EDA), and eye-tracking data. Psychometric assessments captured muscularity concerns (Muscle Dysmorphic Disorder Inventory, MDDI), anxiety (State-Trait Anxiety Inventory, STAI), obsessive-compulsive symptoms (Obsessive-Compulsive Inventory-Revised, OCI-R), appearance-related anxiety (Social Appearance Anxiety Scale, SAAS; Social Physique Anxiety Scale, SPAS), and selfesteem (Self-Liking/Self-Competence Scale-Revised, SLCS-R). The BDD/MD group showed significantly heightened responses to muscular stimuli across all modalities. EEG results revealed increased P300 and late positive potential (LPP) amplitudes, along with greater left-lateralized frontal alpha asymmetry. Eye-tracking indicated longer fixation durations and more frequent fixations on muscular images. EDA results showed elevated levels of autonomic arousal. Between-group differences in psychometric measures were substantial, with large effect sizes. Correlational analyses demonstrated significant associations between MD severity and neurophysiological markers, including LPP amplitude, frontal asymmetry, and EDA reactivity. These findings suggest that men with MD and steroid/PEDs use exhibit amplified attentional, emotional, and physiological reactivity to muscular body images. The integration of EEG, EDA, and eye-tracking provides novel insights into the neurocognitive-affective profile of BDD/MD, emphasizing the salience of idealized physiques in this population. Results support the potential utility of multimodal measures as objective indicators for assessing body image disturbance and underscore the need for targeted interventions addressing perceptual and emotional dysregulation in BDD/MD.
De Novo Malignancy Development Following Kidney Transplantation: Managing Risks and Outcomes in Clinical Practice
(Galenos Publ House, 2025) Huseynov, Amil; Cicek, Sevim Nuran Kuslu
Objective: Denovo malignancy is a significant complication following kidney transplantation, attributed to prolonged immunosuppression.This study evaluates the incidence, risk factors, and clinical outcomes of denovo malignancies in kidney transplant recipients. Material and Methods: A retrospective cohort analysis was conducted on 1200 kidney transplant recipients between 2016 and 2023. Patients were categorized based on the presence or absence of de novo malignancies. Statistical analyses were performed to identify risk factors, including age, sex, comorbidities, and immunosuppressive regimens. Patient and graft survival were assessed using Kaplan-Meier analysis and the log-rank test. Results: Among the study population, 43 patients (3.6%) developed de novo malignancies. The most frequent malignancy types were non-melanoma skin cancers (27.9%) and post-transplant lymphoproliferative disorders (18.6%). Patients with malignancies exhibited a lower three-year survival rate (83.7%) compared to those without malignancies (91.4%), though the difference was not statistically significant (p=0.067). Graft survival at three years was slightly lower in the malignancy group (84.0% vs. 88.7%, p=0.146). Older recipient age was identified as a significant risk factor (hazard ratio=1.03 per year, p=0.025). Conclusion: De novo malignancy remains a concern in kidney transplant recipients, particularly among older patients. Regular screening protocols, lifestyle interventions, and individualized immunosuppressive regimens are essential to mitigate risk and improve outcomes.
Optimized Surgical Outcomes in Living Donor Nephrectomy: A Single-Center Experience with 250 Cases Using a Novel Technique
(Aves, 2025) Huseynov, Amil
Objective: Laparoscopic donor nephrectomy has become a standard of care for living kidney donors, providing reduced morbidity, quicker recovery, and enhanced patient satisfaction compared with open techniques. This study evaluates a modified laparoscopic donor nephrectomy technique designed to minimize colon mobilization while optimizing visualization, thereby improving donor outcomes. Methods: A cross-sectional study of 250 consecutive living donor nephrectomies performed by a single surgeon between March 2022 and March 2024 was conducted. All donors underwent preoperative imaging (3D computed tomography and computed tomographic angiography). The modified approach avoided splenic flexure dissection and introduced early ligation of the adrenal vein. We analyzed intraoperative parameters (operative time, estimated blood loss, complications) and postoperative measures (hospital stay, pain scores, complication rates, satisfaction) were analyzed. Results: The mean operative time was 72.8 +/- 16.2 minutes, with an estimated blood loss of 100 +/- 40 mL. No conversions to open surgery or intraoperative transfusions were required. The mean length of hospital stay was 2.0 +/- 1.0 days. Postoperative pain (mean Visual Analog Scale [VAS] 2.5 +/- 1.8) was low, and major vascular injury occurred in 0.8% of cases. Wound infection and incisional hernia rates were each 0.8%. Patient satisfaction was high (Patient Satisfaction Questionnaire [PSQ] 3.3 +/- 1.4), indicating favorable perceptions of comfort and outcomes. Conclusion: This modified laparoscopic donor nephrectomy technique is safe, effective, and associated with enhanced patient comfort and reduced complications. The findings underscore its potential to improve donor experiences, potentially expanding the living donor pool. Further investigations should confirm these outcomes over a longer follow-up period.
Results in Kidney Transplant Recipients from Living Donors 75 Years of Age or Older
(Elsevier Science Inc, 2024) Cicek, Sevim Nuran Kuslu; Huseynov, Amil; Tuncer, Murat
The increasing age of patients receiving renal replacement therapy (RRT) in Turkey, coupled with a shortage of kidney donors, has led to longer waiting times for transplants and an escalation in mortality rates. This retrospective study aimed to assess the effect on transplant outcomes of accepting kidneys from donors >= 70 years of age, given the rising number of older patients in the population. In all, 1400 patients were transplanted with kidneys from donors >50 years, with patient and graft survival as primary endpoints. Our results demonstrated that the most significant risk factors for graft function were recipient age >65 years, male sex, and presence of type 2 diabetes. Moreover, kidneys from donors >= 75 years of age achieved a half-life of 5 years. These findings suggest that donor age does not necessarily correlate with graft failure and that transplantation from older donors could help alleviate the organ shortage. Further research is needed to substantiate these conclusions.
White-Matter Changes in Early and Late Stages of Mild Cognitive Impairment
(Elsevier Science Ltd, 2020) Femir-Gurtuna, Banu; Kurt, Elif; Ulasoglu-Yildiz, Cigdem; Bayram, Ali; Yildirim, Elif; Soncu-Buyukiscan, Ezgi; Bilgic, Basar
Mild Cognitive Impairment (MCI) is characterized by cognitive deficits that exceed age-related decline, but not interfering with daily living activities. Amnestic type of the disorder (aMCI) is known to have a high risk to progress to Alzheimer's Disease (AD), the most common type of dementia. Identification of very early structural changes in the brain related to the cognitive decline in MCI patients would further contribute to the understanding of the dementias. In the current study, we target to investigate whether the white-matter changes are related to structural changes, as well as the cognitive performance of MCI patients. Forty-nine MCI patients were classified as Early MCI (E-MCI, n = 24) and Late MCI (L-MCI, n = 25) due to their performance on The Free and Cued Selective Reminding Test (FCSRT). Age-Related White-Matter Changes (ARWMC) scale was used to evaluate the white-matter changes in the brain. Volumes of specific brain regions were calculated with the FreeSurfer program. Both group and correlation analyses were conducted to show if there was any association between white-matter hyperintensities (WMHs) and structural changes and cognitive performance. Our results indicate that, L-MCI patients had significantly more WMHs not in all but only in the frontal regions compared to E-MCI patients. Besides, ARWMC scores were not correlated with total hippocampal and white-matter volumes. It can be concluded that WMHs play an important role in MCI and cognitive functions are affected by white-matter changes of MCI patients, especially in the frontal regions. (C) 2020 Elsevier Ltd. All rights reserved.

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