PubMed İndeksli Yayınlar Koleksiyonu

Permanent URI for this collectionhttps://acikerisim2.beykoz.edu.tr/handle/20.500.12879/5

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Design and Simulation of the Microcantilever Biosensor for MITF Antigen and D5 Monoclonal Antibody Interaction Finite Element Analysis, and Experimental
(Bentham Science Publishing Ltd, 2024) Akcali, Pelin; Kelleci, Kubra; Ozer, Sevil
Background Biosensors and MEMS have witnessed rapid development and enormous interest over the past decades. Constant advancement in diagnostic, medical, and chemical applications has been demonstrated in several platforms and tools. In this study, the analytical and FEA of the microcantilever used in biomolecular analyses were compared with the experimental analysis results.Methods In this study, MITF antigen, which is a melanoma biomarker, and anti-MITF antibody (D5) were selected as biomolecules. A MEMS-type microcantilever biosensor was designed by functionalizing the AFM cantilever by utilizing the specific interaction dynamics and intermolecular binding ability between both molecules. Surface functionalization of cantilever micro biosensors was performed by using FEA. The stress that will occur as a result of the interactions between the MITF-D5 has been determined from the deviation in the resonant frequency of the cantilever.Results It has been found that the simulation results are supported by analytical calculations and experimental results.Conclusion The fact that the results of the simulation study overlap with the experimental and mathematical results allows us to get much cheaper and faster answers compared to expensive and time-consuming experimental approaches.
De Novo Malignancy Development Following Kidney Transplantation: Managing Risks and Outcomes in Clinical Practice
(Galenos Publ House, 2025) Huseynov, Amil; Cicek, Sevim Nuran Kuslu
Objective: Denovo malignancy is a significant complication following kidney transplantation, attributed to prolonged immunosuppression.This study evaluates the incidence, risk factors, and clinical outcomes of denovo malignancies in kidney transplant recipients. Material and Methods: A retrospective cohort analysis was conducted on 1200 kidney transplant recipients between 2016 and 2023. Patients were categorized based on the presence or absence of de novo malignancies. Statistical analyses were performed to identify risk factors, including age, sex, comorbidities, and immunosuppressive regimens. Patient and graft survival were assessed using Kaplan-Meier analysis and the log-rank test. Results: Among the study population, 43 patients (3.6%) developed de novo malignancies. The most frequent malignancy types were non-melanoma skin cancers (27.9%) and post-transplant lymphoproliferative disorders (18.6%). Patients with malignancies exhibited a lower three-year survival rate (83.7%) compared to those without malignancies (91.4%), though the difference was not statistically significant (p=0.067). Graft survival at three years was slightly lower in the malignancy group (84.0% vs. 88.7%, p=0.146). Older recipient age was identified as a significant risk factor (hazard ratio=1.03 per year, p=0.025). Conclusion: De novo malignancy remains a concern in kidney transplant recipients, particularly among older patients. Regular screening protocols, lifestyle interventions, and individualized immunosuppressive regimens are essential to mitigate risk and improve outcomes.
Persistent Urinary Tract Infections After Kidney Transplantation: Risk Factors and Management
(Elsevier Inc., 2026) Huseynov A.; Kuşlu Çiçek S.N.; Cicek, Sevim Nuran Kuslu; Kuşlu Çiçek, Sevim Nuran; Huseynov, Amil
Background: Persistent urinary tract infections (UTIs) are a frequent complication among kidney transplant recipients, often leading to increased morbidity, healthcare costs, and potential compromise of graft function. Identifying risk factors and effective management strategies is crucial to improve clinical outcomes in this vulnerable patient population. Methods: In this retrospective study conducted from January 2020 to December 2024, 1130 kidney transplant recipients aged 18 to 75 years were analyzed. Clinical and laboratory data were obtained from electronic medical records, with information on demographics, immunosuppressive regimens, microbiological findings, and treatment outcomes. Persistent UTIs were defined as 2 or more microbiologically confirmed episodes within 12 months despite appropriate therapy. Statistical analyses included descriptive measures, comparative tests, and multivariate logistic regression to identify independent predictors. Results: Of the total cohort, 31.2% developed persistent UTIs, with 68.5% experiencing recurrent episodes. Diabetes mellitus (adjusted OR: 1.56), prolonged urinary catheterization (adjusted OR: 2.04), and infection with multidrug-resistant (MDR) pathogens (adjusted OR: 2.32) emerged as significant risk factors. Escherichia coli was the most frequently isolated organism (36.4%), followed by Klebsiella pneumoniae and Pseudomonas aeruginosa. Patients with persistent UTIs exhibited lower mean eGFR levels, although graft loss rates did not differ significantly from those without persistent UTIs. Conclusion: Persistent UTIs represent a major clinical challenge in kidney transplant recipients. Early identification of modifiable risk factors—particularly glycemic control and appropriate urinary catheter management—may reduce recurrence and preserve renal function. A comprehensive approach involving routine microbiological surveillance and judicious antibiotic use is essential to mitigate the impact of MDR organisms on patient outcomes. © 2026
Regenerative Therapy Approaches and Encountered Problems in Sensorineural Hearing Loss
(Bentham Science Publ Ltd, 2023) Kelleci, Kuebra; Golebetmaz, Eda
Hearing loss is one of the most important public health matters worldwide, severely affecting people's social, psychological, and cognitive development. The perception of sound, movement, and balance in vertebrates depends on a special sensory organ called the cochlea, which contains hair cells and supporting cells in the inner ear. Genetic factors, epigenetics, the use of ototoxic drugs (some antibiotics and chemotherapeutics), noise, infections, or even aging can cause loss of hair cells and their related primary neurons, leading to sensorineural hearing loss. Although a sensorineural hearing loss, also known as permanent hearing loss, is treated with hearing aids and cochlear implants, treatment methods are limited. Since even the best implant cannot exhibit the characteristics of the original ear, the permanent sensory deficit will be permanent. For this reason, it has become important to develop regenerative treatment methods to regenerate and replace lost or damaged hair cells and neurons. Developments in stem cell technology have led to promising studies in regenerating damaged/lost hair cells or neurons with endogenous or exogenous cell-based therapies. Epigenetic mechanisms can turn hearing-related genes on and off and determine which proteins to copy. In addition, due to gene silencing, gene replacement, and CRISPR/CAS9 technology, gene therapy methods have accelerated, and studies have been carried out to treat dominant and recessive mutations that cause genetic-induced hearing loss or increase hair cell regeneration. In this paper, potential gene therapy and stem cell applications in the acquisition of cochlear function, which causes sensorineural hearing loss, and the difficulties encountered in these applications are compiled from a bioengineering perspective.
Results in Kidney Transplant Recipients from Living Donors 75 Years of Age or Older
(Elsevier Science Inc, 2024) Cicek, Sevim Nuran Kuslu; Huseynov, Amil; Tuncer, Murat
The increasing age of patients receiving renal replacement therapy (RRT) in Turkey, coupled with a shortage of kidney donors, has led to longer waiting times for transplants and an escalation in mortality rates. This retrospective study aimed to assess the effect on transplant outcomes of accepting kidneys from donors >= 70 years of age, given the rising number of older patients in the population. In all, 1400 patients were transplanted with kidneys from donors >50 years, with patient and graft survival as primary endpoints. Our results demonstrated that the most significant risk factors for graft function were recipient age >65 years, male sex, and presence of type 2 diabetes. Moreover, kidneys from donors >= 75 years of age achieved a half-life of 5 years. These findings suggest that donor age does not necessarily correlate with graft failure and that transplantation from older donors could help alleviate the organ shortage. Further research is needed to substantiate these conclusions.

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