Cevik, MehtapGunduz, Meliha KoldemirDeliorman, GokceSusleyici, Belgin2021-03-152021-03-1520200301-48510301-48511573-4978https://doi.org/10.1007/s11033-020-05992-5Deliorman, Gokce/0000-0002-3110-5070; Cevik, Mehtap/0000-0001-8912-8307; Susleyici, Belgin/0000-0001-5790-4674Adipocyte death is important in obesity development. Understanding and prevention of adipocyte deaths may be a molecular approach in the treatment. In the study, we aimed to understand role of Niban gene, which acts as an anti-apoptotic molecule as a response to stress conditions, in adipocytes. 3T3-L1 adipocytes were treated with different doses of linoleic acid, hydrogen peroxide and ethanol; and proliferation of the cells examined with real time monitoring iCELLingence system. Gene expression levels were measured by q-PCR. As a response to 24h 480 mu M linoleic acid treatment, Niban gene expression was found to be higher than control group (p = 0.008), whereas 24 h 90 mM ethanol treatment was determined to be lower than control group (p = 0.008). The highest value of Niban gene expression among H2O2 treatment groups was detected in 4h 600 mu M H2O2 in comparison to control group (p = 0.008). To understand role of Niban in adipogenesis, Niban gene expressions were compared between pre-adipocytes and advanced fat accumulated adipocytes and determined to be significantly different (p = 0.042). Our results suggest that Niban might be involved in stress response process in adipocytes. However, the exact molecular role of Niban needs to be investigated in further studies.eninfo:eu-repo/semantics/closedAccessNibanLinoleic AcidCell StressObesityAdipogenesisArticle10.1007/s11033-020-05992-52-s2.0-85095943096